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KMID : 0043319870100030158
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Archives of Pharmacal Research
1987 Volume.10 No. 3 p.158 ~ p.164
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Studies on Protoplast Formation and Regeneration of Ganoderma lucidum
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Choi SH
Kim BK/Kim HW/Kwak JH/Choi EC/Kim YC/Yoo YB/Park YH
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Abstract
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To obtain a new strain of Ganoderma lucidum by protoplast fusion technique, its protoplast formation and regeneration were studied. Several factors affecting the protoplast formation and regeneration were investigated to find their optimum conditions. The mycelium was grown for four days on the cellophane membrane placed on G. lucidum complete medium (GCM). When various commercial lytic enzymes were examined for protoplast isolation, the combination of Novozym 234 and beta-glucuronidase was found to be effective. An osmotic stabilizer, 0.6M sucrose in 20mM phosphate buffer pH 5.8, gave the highest yield of protoplasts. Three-hour incubation in shaking incubator was most suitable for releasing protoplasts. To increase the protoplast yield, pretreatment with 2-mercaptoethanol was carried out. The regeneration frequency in GCM containing 0.6M MgSO4.7H20 Was shown to be 0.66%.
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